Development of chemically defined media supporting high-cell-density growth of lactococci, enterococci, and streptococci.

P-136: In Vitro Development of Ovine Oocytes Matured in A Chemically Defined Medium

Background: Despite great advances achieved in the field of in vitro oocyte maturation (IVM), almost all IVM media are supplemented with undefined component which not only pose the risk of pathogen transmission but also hinders our knowledge of molecular mechanisms of maturation. One of these components is serum that containing various concentration of unknown molecules affecting cellular proce...

media and specific growth rate selection for high-cell-density cultivation of recombinant escherichia coli producing hGM-CSF in fed-batch process

The Seventh International Conference on the Genetics of Streptococci, Lactococci, and Enterococci.

Medium development beyond production media: Chemically defined media for transfection and single cell cultivation

Background State-of-the-art medium and feed development has proven its potential for increasing bioprocess efficiency many times. By the application of such advanced chemically defined and animal component-free formulations, in combination with recent cell lines, the yield of mammalian cell cultures was pushed to the binary gram per liter level. In this work, the current progress in designing s...

Enhancement of Alpha 1-antitrypsin Production in Pichia pastoris by Designing and Optimizing Medium Using Elemental Analysis

Background: Human alpha 1-antitrypsin (AAT) is a monomeric glycosylated protein; it is the potent inhibitor of a whole range of serine proteases and protects tissues against their destructive effects. The human plasma-derived AAT, which is currently used to augment the AAT level in patients, is limited due to high cost and source limitation. Recombinant production of AAT can be considered as a ...